A water-soluble polysaccharide from barley leaves

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Purification and Characterization of Three Soluble Invertases from Barley ( Hordeum vulgare 1 . ) Leaves '

Three soluble isoforms of invertase (8-fructofuranosidase; EC 3.2.1.26) were purified from 7-d-old primary leaves of barley (Hordeum vurgare 1.). lnvertase I, a monomeric protein of 64 kD, was purified to apparent homogeneity as shown by sodium dodecylsulfate-polyacrylamide gel electrophoresis. lnvertases IIA and IIB, multimeric proteins with molecular masses of 116 and 155 kD, were purified 78...

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An edible fungal polysaccharide termed as ABP was obtained by extraction with hot water, and followed successive chromatographic purification using DEAE-Sepharose Fast Flow column and Sephacryl S-300 High-Resolution column. A symmetrical peak was obtained on high-performance size-exclusion chromatography with an average molecular weight of 5.17 × 104 Da, which was named ABP, and its main compon...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1937

ISSN: 0306-3283

DOI: 10.1042/bj0310786